Matthias Hardtke Wolenski, Speaker at Immunology Conferences
Hannover Medical School, Germany
Title : Regulatory T cells with chimeric antigen receptors as treatment for autoimmunity

Abstract:

Adoptive immunotherapy with ex vivo expanded, polyspecific regulatory T cells (Tregs) is a promising treatment for graft-versus-host disease. Animal transplantation models used by us and others have demonstrated that the adoptive transfer of allospecific Tregs offers greater protection from graft rejection than that of polyclonal Tregs. This finding is in contrast to those of autoimmune models, where adoptive transfer of polyspecific Tregs had very limited effects, while antigen-specific Tregs were promising. However, antigen-specific Tregs in autoimmunity cannot be isolated in sufficient numbers.

Chimeric antigen receptors (CARs) can modify T cells and redirect their specificity toward needed antigens and are currently clinically used in leukemia patients. A major benefit of CAR technology is its “off-the-shelf” usability in a translational setting in contrast to major histocompatibility complex (MHC)-restricted T cell receptors.

We used CAR technology to redirect T cell specificity toward insulin and redirect T effector cells (Teffs) to Tregs by Foxp3 transduction. Our data demonstrate that our converted, insulin-specific CAR Tregs (cTregs) were functional stable, suppressive and long-lived in vivo.

This is a proof of concept for both redirection of T cell specificity and conversion of Teffs to cTregs.

Biography:

  • Research group leader in the Department of Gastroenterology, Hepatology and Endocrinology at Hannover Medical School since 2021
  • Research group leader in the Institute of Medical Microbiology at University Medicine Essen since 2021
  • Head of Research in the Department of Gastroenterology and Hepatology at University Medicine Essen from 2018-2021

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