Title : Up regulation of treg cells in peripheral blood of non-small cell lung cancer and the analysis of treg related long non-coding RNA
Abstract:
Objective: To investigate changes of regulatory T cells (Treg) in non-small cell lung cancer (NSCLC) and their interaction with long non-coding RNA(lnc RNA).
Methods: A total of 20 patients with non-small cell lung cancer admitted during January 2019 and August 2019 were chosen as carcinoma group, and 30 healthy people taking medical examination at the same period were chosen as control group. Serum Treg accounting for CD4+T cell proportions were monitored by flow cytometry and Forkhead/winged helix family transcription factor3 (Foxp3) were monitored by reverse-transcription PCR. Screening of differentially expressed genes by Agilent Cerna microarray in 3 pairs of lung cancer patients and healthy controls and analysis of lnc RNA associated with Foxp3 mRNA by Bioinformatics.
Results: Compared with those in control group, Treg accounting for CD4+T cell proportions and relative expression of Foxp3 mRNA were significantly increased in carcinoma group(t=7.29?P?0.05; t=21.59?P?0.05). A total of 4626 differential genes were found in 3 pairs of lung cancer group and healthy control group. Among these genes, 2095 were up-regulated, 2531 were down regulated, and Foxp3 was up-regulated. With Foxp3 as the target, a series of differentially expressed lnc RNA related to Foxp3 were found by using transcription factors to predict Foxp3 relatedlnc RNA. Among them, the lnc RNA NONHSAT197842.1 showed the most significant difference (6.25 times).
Conclusion: The increase of Treg in NSCLC may be related to lnc RNA. Treg may be used as a molecular marker for screening of lung cancer, and lnc RNA NONHSAT197842.1 may provide a new target for precise treatment of lung cancer.
